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Cell Biologist
Cell BiologistGlobalchannelmanagement • Boston, New Hampshire, USA
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Cell Biologist

Cell Biologist

Globalchannelmanagement • Boston, New Hampshire, USA
30+ days ago
Job type
  • Full-time
Job description

Cell Biologist needs 3 years for MS - 5 years for BS. Extensive hands-on experience with human iPSC culture gene editing and molecular biology techniques.

Cell Biologist requires :

  • Proficiency in gene editing and cell line development including optimization of protocols familiarity with flow-based sorting.
  • Hands-on experience with cell delivery methods of gene editing tools.
  • Excellent communication and interpersonal skills with the ability to work effectively in a team-oriented environment.
  • Strong organizational skills and diligent record keeping.
  • Experience in pancreatic islet directed differentiation is highly desirable
  • Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable.
  • Mandatory weekend cell culture responsibilities on rotation basis.

Cell Biologist duties :

  • Enabling and advancing iPSC gene editing cell line development and evaluation of differentiated cells.
  • Routine iPSC cell culture passaging and media making.
  • Optimize workflows for iPSC CRISPR based gene-editing gRNA screening and delivery.
  • Optimize and perform assays for on- / off-target analysis via PCR and NGS workflows.
  • Generate clonal gene edited iPSC lines and characterize via flow cytometry ddPCR dPCR and qPCR.
  • Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts viability metabolite assessments.
  • Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance.
  • Collaborate cross-functionally to support transfer of R&D assays to GMP.
  • Maintain accurate and detailed records of all experiments synthesize results prepare reports and present in meetings.
  • Contribute to SOP development.
  • Work seamlessly in executing workplans and meeting deadlines.
  • Key Skills

    Laboratory Experience,Transfection,Bioinformatics,DNA Isolation,Genetics,PCR,R,Biochemistry,Next Generation Sequencing,Cell Culture,Molecular Biology,Research Laboratory Experience

    Employment Type : Full Time

    Experience : years

    Vacancy : 1

    Hourly Salary Salary : 53 - 54

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    Biologist • Boston, New Hampshire, USA

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