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Lab Technician I

Lab Technician I

Genesis Global GroupHamilton Twp, NJ, United States
1 day ago
Job type
  • Full-time
Job description

Laboratory Technician

Job Summary : Medical Diagnostic Laboratories (MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.

MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).

MDL is looking for a Laboratory Technician. The lab technician is responsible for accessioning and processing specimens, performing automated and manual extractions, Real-Time and Conventional PCR, gel electrophoresis, Pyrosequencing, cell culture, and Next-Generation sequencing.

Essential Functions :

Accessioning :

  • Print labels and attach to requisitions and the corresponding specimens
  • Properly accession multiple specimen types
  • Take photographs of all specimens received
  • Properly identify any discrepant information
  • File all paperwork in order and distribute to the appropriate QC bins
  • Properly scan accessioned requisitions
  • Scan 2-D barcode elution tubes used for extraction
  • Properly locate and record temperatures for all necessary stations

Manual Extraction :

  • Perform Phenol / Chloroform manual extractions on biopsies and ticks
  • Properly separate samples into their organic and inorganic layers via phenol / chloroform
  • Properly perform quantitative analysis and hydrate all ticks and biopsies
  • Robotic Extraction :

  • Properly extract all samples types using a provided XG sheet as a template
  • Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
  • Properly dispose of all waste material when required; both hazardous and non-hazardous
  • Attend to robotics to address any user interactions and / or robotic malfunctions
  • Manually extract all necessary samples via commercial kits
  • RNA Extraction :

  • Perform manual RNA extractions via Qiagen kit for all necessary samples
  • Perform robotic RNA extractions via VX Reagents kit for all necessary samples
  • PCR :

  • Perform manual and robotic Conventional method PCR amplification
  • Properly load and start thermocycler machines
  • Perform manual and robotic Real-time method PCR amplification
  • Properly load and start Rotor-gene thermocycler
  • Perform initial analysis on completed Real-time PCR
  • Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
  • Properly load and start Bio-Rad CFX thermocycler
  • Perform initial analysis on completed Acoustic Real-Time PCR
  • Gel Electrophoresis :

  • Properly identify which completed PCRs from the thermocycler require gel electrophoresis
  • Properly identify when to make and / or use 2% versus 2.5% gels
  • Complete blank gel template sheets to combine short PCR sheets
  • Use dye to stain all necessary samples with the appropriate volume on a test by test basis
  • Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
  • Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
  • Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors
  • BioPlex Amalysis

  • Properly perform MagPlex PCR
  • Properly remove any unused dNTPs and primers within the amplified PCR product with the use of ExoSAP-IT
  • Properly perform Single Base, and Allele Specific Extension
  • Properly attach samples to appropriate bead mixtures, and subsequently label samples with Strptavidin-R-phycoerythrin (SAPE)
  • Properly load and start BioPlex machine
  • Properly analyze all run data
  • Micro-Biology / Cell Culture

  • Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies
  • Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs
  • Properly analyze growth plate to determine antibiotic susceptibility or resistance
  • For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System
  • Identify and pick colonies for PCR
  • Perform CAMP test and analyze for zones of hemolysis
  • Next-Generation Sequencing

  • Utilize the Illumina platform to test for BRCA, Pharmacogenomics, and Cystic Fibrosis
  • Properly perform a series of nested PCRs
  • Properly attach samples to appropriate bead mixtures
  • Properly perform bead cleaning to remove unused dNTP's and primers within the amplified PCR product
  • Properly perform gel purification
  • Properly load and operate multiple Life Technologies and Illumina equipment
  • Perform Sanger Sequencing
  • Properly analyze / export all run data
  • Additional Responsibilities :

  • Performs other similar or related duties as required or assigned.
  • Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
  • Assures customer privacy is maintained at all times.
  • Participates as a team member and is accountable for own work responsibilities.
  • Assures information is delivered in a manner that is supportive, timely and understandable.
  • Initiative is demonstrated to proactively diagnose and resolve problems.
  • Productive work habits are consistently displayed.
  • Accuracy and quality are maintained for all activities.
  • Job Qualifications : General K nowledge, S kills, and A bilities (KSA's) required

  • 9 month Clinical Laboratory Technician training
  • Physical Demands : Physical, Mental and workplace Environment Conditions

  • Ability to sit, stand, walk and perform repetitive motion (manual pipette)
  • Proficient visual and audio skills
  • Working Conditions : Workplace Environment Conditions

  • Laboratory setting
  • Use of personal protective equipment
  • Education and Experience :

  • Bachelor Degree in Biology, Chemistry, or related science field
  • We Offer :

  • Competitive Salary
  • Medical, Dental & Vision Insurance
  • Short and Long Term Disability
  • Life Insurance
  • Dependent Care Flex Spending Account
  • Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)
  • Identity Theft Protection
  • 401(k) Plan
  • Paid Vacation & Holiday
  • Business Casual Environment
  • Visit us at

    Medical Diagnostic Laboratories is a member of Genesis Global Group, an equal opportunity employer.

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    Lab Technician • Hamilton Twp, NJ, United States

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