Laboratory Technician
Job Summary : Medical Diagnostic Laboratories (MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.
MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).
MDL is looking for a Laboratory Technician. The lab technician is responsible for accessioning and processing specimens, performing automated and manual extractions, Real-Time and Conventional PCR, gel electrophoresis, Pyrosequencing, cell culture, and Next-Generation sequencing.
Essential Functions :
Accessioning :
- Print labels and attach to requisitions and the corresponding specimens
- Properly accession multiple specimen types
- Take photographs of all specimens received
- Properly identify any discrepant information
- File all paperwork in order and distribute to the appropriate QC bins
- Properly scan accessioned requisitions
- Scan 2-D barcode elution tubes used for extraction
- Properly locate and record temperatures for all necessary stations
Manual Extraction :
Perform Phenol / Chloroform manual extractions on biopsies and ticksProperly separate samples into their organic and inorganic layers via phenol / chloroformProperly perform quantitative analysis and hydrate all ticks and biopsiesRobotic Extraction :
Properly extract all samples types using a provided XG sheet as a templateProperly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagentsProperly dispose of all waste material when required; both hazardous and non-hazardousAttend to robotics to address any user interactions and / or robotic malfunctionsManually extract all necessary samples via commercial kitsRNA Extraction :
Perform manual RNA extractions via Qiagen kit for all necessary samplesPerform robotic RNA extractions via VX Reagents kit for all necessary samplesPCR :
Perform manual and robotic Conventional method PCR amplificationProperly load and start thermocycler machinesPerform manual and robotic Real-time method PCR amplificationProperly load and start Rotor-gene thermocyclerPerform initial analysis on completed Real-time PCRPerform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic DispensorProperly load and start Bio-Rad CFX thermocyclerPerform initial analysis on completed Acoustic Real-Time PCRGel Electrophoresis :
Properly identify which completed PCRs from the thermocycler require gel electrophoresisProperly identify when to make and / or use 2% versus 2.5% gelsComplete blank gel template sheets to combine short PCR sheetsUse dye to stain all necessary samples with the appropriate volume on a test by test basisProperly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)Properly capture a visual image of the gel with the use of a SYNGENE UV gel readerBe able to troubleshoot any gel box or power source errors, as well as any gel reader errorsBioPlex Amalysis
Properly perform MagPlex PCRProperly remove any unused dNTPs and primers within the amplified PCR product with the use of ExoSAP-ITProperly perform Single Base, and Allele Specific ExtensionProperly attach samples to appropriate bead mixtures, and subsequently label samples with Strptavidin-R-phycoerythrin (SAPE)Properly load and start BioPlex machineProperly analyze all run dataMicro-Biology / Cell Culture
Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single coloniesProperly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discsProperly analyze growth plate to determine antibiotic susceptibility or resistanceFor GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber SystemIdentify and pick colonies for PCRPerform CAMP test and analyze for zones of hemolysisNext-Generation Sequencing
Utilize the Illumina platform to test for BRCA, Pharmacogenomics, and Cystic FibrosisProperly perform a series of nested PCRsProperly attach samples to appropriate bead mixturesProperly perform bead cleaning to remove unused dNTP's and primers within the amplified PCR productProperly perform gel purificationProperly load and operate multiple Life Technologies and Illumina equipmentPerform Sanger SequencingProperly analyze / export all run dataAdditional Responsibilities :
Performs other similar or related duties as required or assigned.Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.Assures customer privacy is maintained at all times.Participates as a team member and is accountable for own work responsibilities.Assures information is delivered in a manner that is supportive, timely and understandable.Initiative is demonstrated to proactively diagnose and resolve problems.Productive work habits are consistently displayed.Accuracy and quality are maintained for all activities.Job Qualifications : General K nowledge, S kills, and A bilities (KSA's) required
9 month Clinical Laboratory Technician trainingPhysical Demands : Physical, Mental and workplace Environment Conditions
Ability to sit, stand, walk and perform repetitive motion (manual pipette)Proficient visual and audio skillsWorking Conditions : Workplace Environment Conditions
Laboratory settingUse of personal protective equipmentEducation and Experience :
Bachelor Degree in Biology, Chemistry, or related science fieldWe Offer :
Competitive SalaryMedical, Dental & Vision InsuranceShort and Long Term DisabilityLife InsuranceDependent Care Flex Spending AccountVoluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)Identity Theft Protection401(k) PlanPaid Vacation & HolidayBusiness Casual EnvironmentVisit us at
Medical Diagnostic Laboratories is a member of Genesis Global Group, an equal opportunity employer.