Laboratory Technician II / III

Overview

Our client is a biotechnology company that uses the MygoGenesis TM proprietary process to turn somatic cells into totipotent cells, and then to any cell or tissue, faster, more reliably, more efficiently, and with none of the challenges of alternative methods. Products developed using the Mygotic Process TM hold enormous potential to revolutionize cell and gene therapies, food and water testing, and regenerative medicine. These new products will open new markets and enable safer drugs, other products and consumables for humans and animals.

Our client is looking to hire a Research Laboratory Technician with 2 to 4 years of cell culture and laboratory experience in culturing and expanding crab, mammalian, and human primary and commercial cell lines in 2D and 3D systems, followed by characterization, imaging, banking, cryopreservation, and thawing techniques as an independent contractor. The candidate will be working in a fast-paced environment on short- and long-term laboratory assignments with various scientific complexities. The potential candidate is expected to be motivated and efficient when working in a challenging and highly rewarding research environment.

They will be responsible for the performance of operations in support of starting / establishing, growth, and production of naïve embryonic stem cells, hESCs, iPSCs, and differentiated cells from tissue samples under GLP and GMP conditions and other industrial and federal regulations as required. These functions may include expansion and maintenance of ESCs, propagation and differentiation of ESCs into other cell lines in culture flasks and bioreactors using aseptic techniques, cryopreservation of cells, preparation of media and reagents, filtration, inventory management, microscopy techniques, collecting and analyzing data, and preparing reports. Other laboratory assignments may include assistance in genetic modification of cells, protein extractions for SDS-PAGE and activity studies, and DNA and RNA extractions for using qPCR and sequencing (RNA-Seq) analyses . Routine activities may include performing basic pH measurements and preparing chemical reagents at specific molar concentrations . The candidate is expected to be able to work independently and as part of a team, summarize and present data at a professional level, as well as suggest creative ways to troubleshoot the issues encountered or provide alternatives.

Responsibilities :

• Interact and communicate effectively with the supervisor and other site personnel.
• Assist in the preparation and analysis of laboratory experiments, as requested.
• Perform advanced cell culture techniques, including 2D and 3D cell culturing, media formulation and preparation, cell passaging, counting, and staining, cryopreservation, maintenance, and monitoring of cell cultures, ensure optimal growth conditions, and other related bioassays of cell cultures.
• Perform molecular cell biology techniques, such as nucleic acid and protein extraction and purification, for bioanalytical assays, and read / analyze data.
• Capable of culturing tissues and isolating cells under sterile conditions.
• Perform preliminary research, information gathering, and information packaging.
• Collect diverse data, document, and present it professionally to senior management.
• Assist the laboratory team in the design and execution of experiments, optimization of protocols, and the collection of data and data input.
• Document all lab procedures, correspondence, and communication pertinent to the research.
• Adhere to Occupational Safety and Health Administration (OSHA), Good Laboratory Practices (GLP), Good Manufacturing Practices (GMP), safety, and infection control procedures.
• Responsible for cleaning, disinfecting, and maintaining all laboratories and equipment.
• Assist with receipt and inspection of raw materials, lab ordering, and stocking, as requested.
• Process and ship all samples according to regulations and protocol requirements.
• Maintain and update files, SOPs, protocols, and all applicable regulatory documentation.
• Self-motivated and detail-oriented with a proven ability to learn quickly.

Qualifications :

Education :