Staff Research Associate III - Ophthalmology

Description

Under the

direction of the Principal Investigator (PI), the Staff Research Associate III

(SRA III) is responsible for executing cellular and molecular techniques as

part of the basic, translational and clinical research. Duties include the cell

culture of primary cells and cell lines as well as other molecular biology

techniques including but not limited to cloning, mutagenesis, sequencing,

polymerase chain reaction (PCR), Western Blot, immunohistochemistry / immunocytochemistry,

and cryosectioning. The SRA III will perform imaging acquisition and imaging

and data analysis, keep good documentation records in compliance, assist with

pre-clinical experiments and actively participate in qualifications and

clinical manufacturing part of a Phase I clinical trial.

The SRA III

will also be in charge of lab management and lab maintenance including but not

limited to overseeing lab activities ensuring that a safe, clean, organized,

well equipped / stocked and compliant environment is maintained.

Qualifications

All

Required : Bachelor's

degree in life science (not including psychology) and or equivalent experience

and / or training

Knowledge of

molecular biology techniques

Skills and

extensive experience in human and mouse cell culture technique

Knowledge of

cell culture, including thawing cells, culturing cells and freezing cells

Skills in

cryosection

Skills in

isolating and purifying high quality DNA and RNA

Skill in

subcloning of cell lines

Ability to use

molecular biology software

Ability and

experience to perform immunohistochemistry

Ability to

perform protein analysis by gel electrophoresis and Western Blots

Experience with

tissue and cell imaging and analysis

Ability to

establish and maintain records and supply stocks

Skill in

maintaining the lab and equipment in working condition

Ability to

establish and maintain cooperative working relationships with co-workers

Demonstrated

experience using general lab equipment such as pipettes, centrifuges,

balances,incubators, thermal cyclers, dry blocks, shakers, autoclaves,

water baths, liquid nitrogen tanks,DNA synthesizer, pH meter, etc.

Ability to work

with mice, setting up breeding pairs and recording births and weaning animals

Ability to

plan, execute and coordinate all the GMP / GLP activities related to the

pre-clinicaland Phase I clinical projects in collaboration with the

research teams

Knowledge of

GLP and GMP

Ability to

manage and maintain the lab ensuring that a safe, clean and compliant

environment ismaintained

Ability to work

on multiple projects, often changing from one task to another

Ability to

establish and maintain cooperative working relationships with personnel both in

andoutside of the laboratory group

Communicate

clearly, concisely and accurately in verbal and written formats

Ability to

summarize and present data