Molecular and Cellular Biologist

Job Title : Molecular and Cellular Biologist Location : Cambridge, MA Type : Contract Overview Leading pharmaceutical company looking for an experienced Molecular and Cellular Biologist. Ideal candidates should have a BS or MS and 3+ years of hands on experience with Molecular biology techniques including qPCR, and gene editing. Responsibilities Generate custom lentiviral libraries and maintain extensive cell cultures. Generate edited pools of cells via lentiviral transduction and arrayed electroporation. Generate single-cell data via several commercial workflows, including from 10x Genomics. Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale. Requirements EDUCATION AND EXPERIENCE : Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field TECHNICAL SKILLS REQUIREMENTS : 3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry. Bonus : direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing. Benefits System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan. Ref : #558-Scientific