Scientist (T Cells/Flow Cytometry)

Scientist (T Cells/Flow Cytometry)

Russell Tobin is partnering with a leading biotechnology organization to identify a Scientist to support Discovery Biology research and binder screening for T-cell-based therapies. This role focuses on bench-level experimentation, non-viral T-cell engineering, and functional validation of therapeutic candidates.

Responsibilities

· Perform non-viral engineering of primary human T cells using mRNA or transposon-based DNA delivery systems and electroporation or nucleofection platforms.

· Monitor cell viability, recovery, and synthetic receptor expression following transfection.

· Maintain and expand primary human T cells and Jurkat cell lines using aseptic laboratory techniques.

· Execute high-throughput screening assays to evaluate binder affinity, specificity, potency, and functionality.

· Perform multi-color flow cytometry (FACS) to assess surface expression and phenotypic markers, analyzing results using FlowJo.

· Conduct T-cell activation and cytotoxicity assays using IncuCyte live-cell imaging or LDH-release methods.

· Perform cytokine profiling using ELISA, MSD, or Luminex platforms and assess T-cell proliferation using dyes such as CFSE.

· Record experimental procedures, raw data, and protocol deviations accurately in an Electronic Lab Notebook (ELN).

· Analyze experimental results, troubleshoot assay workflows, and provide data-driven updates during weekly campaign meetings.

· Collaborate with Discovery Biology team members to advance binder screening and functional validation activities.

Requirements

· Bachelor's or Master's degree in Immunology, Molecular Biology, Bioengineering, or a related biological science.

· Minimum 2 years of hands-on wet lab experience, preferably in an industry setting.

· Strong aseptic technique and experience isolating primary T cells from peripheral blood mononuclear cells (PBMCs).

Expertise in flow cytometry acquisition and data analysis is required.

· Experience with primary T-cell culture, cell-based assays, and immunological functional testing.

· Familiarity with non-viral transfection methods, including electroporation or nucleofection, preferred.

· Experience with cytotoxicity assays, cytokine analysis, and T-cell proliferation assays preferred.

· Experience using liquid-handling robotics for high-throughput screening is a plus.

· Strong documentation, analytical, communication, and problem-solving skills.

· Ability to work independently at the bench in a fast-paced drug discovery environment.

Additional Details:

• Location model: On-site — San Diego, CA
• Schedule: 1st shift, standard business hours
• Position Type: 7–8-month contract with the possibility of extension
• Pay range: $45–$48/hr, depending on experience.

APPLY NOW!

Russell Tobin is a professional recruitment and staffing advisory firm that connects job seekers and employers across various industries. We are a staffing arm of Pride Global. Affiliates include Russell Tobin, Pride Health, Pride Now and Pride One.

For workers in the United States, PrideGlobal and it's affiliates offers eligible employee's comprehensive healthcare coverage (medical, dental, and vision plans), supplemental coverage (accident insurance, critical illness insurance and hospital indemnity), 401(k)-retirement savings, life & disability insurance, an employee assistance program, legal support, pet insurance and employee discounts with preferred vendors.

Equal Employment Opportunity

PrideGlobal and it's affiliates are an equal opportunity employer. We do not discriminate on the basis of the race, religious creed, color, national origin, ancestry, physical disability, mental disability, reproductive health decision making, medical condition, genetic information, marital status, sex, gender, gender identity, gender expression, age, sexual orientation, veteran or military status, or any other characteristic protected by applicable federal, state, or local law.

Fair Chance Employment

PrideGlobal and it's affiliates are a Fair Chance employer. We consider all qualified applicants, including those with criminal histories, in a manner consistent with applicable state and local Fair Chance laws and ordinances, including, the California Fair Chance Act and all applicable local Fair Chance ordinances.

Accommodations

We are committed to providing reasonable accommodations to applicants and employees with disabilities. If you require a reasonable accommodation to participate in the application or interview process, or to perform the essential functions of this role, please contact us.

Only applicable for San Francisco Candidates Under the San Francisco Lactation in the Workplace Ordinance, we will provide written notice of lactation accommodation rights, and this notice will automatically be given upon hiring, any inquiry of parental leave or lactation accommodation.

Only applicable for Kentucky positions: For this type of employment, state law requires a criminal record check as a condition of employment. This applies to direct care staff (Examples: RN, LPN, Nurse Aides, Therapists) referred to Kentucky nursing facilities, assisted living communities, or long-term care facilities, in accordance with KRS 216.793.