Scientist

Title : Scientist

Location : Cambridge-MA

Duration : 18 months

Job Description :

• Biotherapeutic Expression and Purification group (BEP).
• Essential role in both RNA production and transient protein production research laboratories.
• The BEP production groups are looking for a laboratory technician to provide DNA, RNA and protein production support for the RNA and protein activities in BEP.
• The candidate is expected to have a background in biochemistry techniques including nucleic acids, protein purification, and cell culture.
• The successful candidate will grow bacterial and transient cultures, perform large-scale DNA production activities, synthesize RNA, and purify protein following standardized protocols.
• The candidate will be a key contributor to the scientific team responsible for RNA and protein production and will report to the head of RNA Production.
• Conduct routine end-to-end molecular biology and biochemistry techniques.
• Cell transformations, day cultures, bacterial cultures, plasmid purification and digestion.
• DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS).
• In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing.
• Transient cell culture techniques such as transfection, cell number and viability monitoring, cell lysis and protein harvest
• Purification of proteins using liquid chromatography techniques.
• Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing.
• Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using Microsoft products and an online database for request management.
• Maintaining reagent, DNA, RNA and protein inventory.

Qualifications :

• Proficiency in biotechnology laboratory techniques and performing quantitative analysis.
• Experience working with bacterial cell lines and cell culture.
• Knowledge of nucleic acid purification and synthesis.
• Knowledge of protein purification techniques.
• Proficiency in biotechnology laboratory techniques and performing quantitative analysis.
• Knowledge of nucleic acid purification and synthesis.
• Knowledge of protein purification techniques

Preferred Skills :

• Experience working with bacterial cell lines and cell culture.
• In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing.
• Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing.
30+ days ago