Technician B - Cardiology
Columbia University
Columbia University Medical Center
$51.8K a year
Full-time
Position Summary
Technician B will provide bench and data analysis support for cell biology, molecular biology, immunology, and functional genomic assays, and assist the lab team with experiments and general research activities.
Responsibilities
- Will be responsible for assuring compliance with study protocols, including IACUC and IRB, and laboratory safety.
- Will perform routine cellular and molecular assays (e.g., transformation, miniprep, maxiprep, RNA, DNA and protein preparation, cDNA synthesis, qRT-PCR, transfection, packaging of viral vectors, ELISA, Western Blotting, etc.).
- Will maintain primary mouse and human cell culture and human induced pluripotent stem cell culture and differentiation.
- Will utilize microscopic techniques and FACS for characterization / functional assays of cell lines and primary cells.
- Will perform histological sectioning, staining, imaging, and quantitative data analysis.
- Assist with other lab team efforts, including breeding, genotyping, and preparation of animal models for experiments and surgeries.
- Assist with sample preparation of RNA-seq, single cell RNA-seq, CRIPSR screening, high content imaging, and other functional genomic assays.
- Interpreting and presenting data, and participating in manuscript preparation.
- Ordering and maintaining laboratory supplies, chemicals, and equipment.
- Performing other duties as required.
Minimum Qualifications
A Bachelor’s degree in biology, chemistry, biochemistry, molecular biology, biostatistics, or other biomedical research related fields or equivalent in education training and experience.
Preferred Qualifications
- Experiences in a research laboratory
- Familiar with cell biology, molecular biology, immunology, and functional genomic techniques, and animal models
- Capable of developing skills in experimental design, data analysis, visualization, and interpretation.
1 day ago