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Technician B - Cardiology

Columbia University
Columbia University Medical Center
$51.8K a year
Full-time

Position Summary

Technician B will provide bench and data analysis support for cell biology, molecular biology, immunology, and functional genomic assays, and assist the lab team with experiments and general research activities.

Responsibilities

  • Will be responsible for assuring compliance with study protocols, including IACUC and IRB, and laboratory safety.
  • Will perform routine cellular and molecular assays (e.g., transformation, miniprep, maxiprep, RNA, DNA and protein preparation, cDNA synthesis, qRT-PCR, transfection, packaging of viral vectors, ELISA, Western Blotting, etc.).
  • Will maintain primary mouse and human cell culture and human induced pluripotent stem cell culture and differentiation.
  • Will utilize microscopic techniques and FACS for characterization / functional assays of cell lines and primary cells.
  • Will perform histological sectioning, staining, imaging, and quantitative data analysis.
  • Assist with other lab team efforts, including breeding, genotyping, and preparation of animal models for experiments and surgeries.
  • Assist with sample preparation of RNA-seq, single cell RNA-seq, CRIPSR screening, high content imaging, and other functional genomic assays.
  • Interpreting and presenting data, and participating in manuscript preparation.
  • Ordering and maintaining laboratory supplies, chemicals, and equipment.
  • Performing other duties as required.

Minimum Qualifications

A Bachelor’s degree in biology, chemistry, biochemistry, molecular biology, biostatistics, or other biomedical research related fields or equivalent in education training and experience.

Preferred Qualifications

  • Experiences in a research laboratory
  • Familiar with cell biology, molecular biology, immunology, and functional genomic techniques, and animal models
  • Capable of developing skills in experimental design, data analysis, visualization, and interpretation.
  • 1 day ago
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