Tissue Culture Lab Technician (1st Shift)

Job Description

Job Description

Position : Tissue Culture Lab Technician

Shift : 1st Shift (7am - 3pm)

Location : South San Francisco

Type : Full-time

Desired start date : ASAP

The Opportunity :

Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands-on work, and responsible. Prior experience in a sterile work environment, plant breeding or gene editing is a plus, but is not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role will involve additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work, and offers opportunities to contribute to various aspects of our lab's growth.

Responsibilities :

• Protoplast Isolation, Transfection, and Culture :
• Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts.
• Transfect protoplasts using PEG-mediated transfection or electroporation methods.
• Culture transfected protoplasts to regenerate callus
• Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
• Microscopy and Imaging :
• Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
• Data Recording and Analysis :
• Maintain accurate and detailed electronic lab notebooks (ELN) and batch records.
• Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
• Sample Preparation :
• Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
• Cross-Functional Support and Collaboration :
• Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.

Lab Maintenance and Compliance :

Candidate Profile :

Desired skills / experience :

Below is the pay range for this position that we reasonably expect to pay. Individual compensation is based on various factors including experience, education, skillset, and other relevant qualifications. This range is for the South San Francisco, California location and may be adjusted to the labor market in other geographic areas.

Anticipated Compensation : $29.50 / hr

A bout Ohalo Genetics :

Ohalo accelerates evolution to unlock nature's potential. We use technology to transform agriculture providing farmers new plant varieties with unparalleled yield, efficiency, and profitability while shrinking the land, water and energy footprint of our crops.

Notes : If you previously applied for a job at Ohalo Genetics, we encourage you to restate your interest in the position by submitting your application.

No visa sponsorship is available for this position at this time.

No recruiters, please.